Computational coherent Raman scattering imaging: breaking physical barriers by fusion of advanced instrumentation and data science
نویسندگان
چکیده
Abstract Coherent Raman scattering (CRS) microscopy is a chemical imaging modality that provides contrast based on intrinsic biomolecular vibrations. To date, endeavors instrumentation have advanced CRS into powerful analytical tool for studies of cell functions and in situ clinical diagnosis. Nevertheless, the small cross-section sets up physical boundary design space system, which trades off speed, signal fidelity spectral bandwidth. The synergistic combination computational approaches offers way to break trade-off. In this review, we first introduce coherent recent developments, then discuss current methods, including compressive micro-spectroscopy, volumetric imaging, as well machine learning algorithms improve system performance decipher information. We foresee constant permeation concepts push capability microscopy.
منابع مشابه
Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications
Coherent anti-Stokes Raman scattering (CARS) microscopy permits vibrational imaging with high-sensitivity, high speed, and three-dimensional spatial resolution. We review recent advances in CARS microscopy, including experimental design, theoretical understanding of contrast mechanisms, and applications to chemical and biological systems. We also review the development of multiplex CARS microsp...
متن کاملThree-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
Confocal [1] and multiphoton [2] fluorescence microscopy have become powerful techniques for threedimensional imaging of chemical and biological samples, especially for live cells. This coincides with developments of various natural and artificial fluorescent probes for cellular constituents [3]. For chemical species or cellular components that either do not fluoresce or cannot tolerate labelin...
متن کاملHeterodyne coherent anti-Stokes Raman scattering (CARS) imaging.
We have achieved rapid nonlinear vibrational imaging free of nonresonant background with heterodyne coherent anti-Stokes Raman scattering (CARS) interferometric microscopy. This technique completely separates the real and imaginary responses of nonlinear susceptibility chi(3) and yields a signal that is linear in the concentration of vibrational modes. We show that heterodyne CARS microscopy pe...
متن کاملBiological imaging with coherent Raman scattering microscopy: a tutorial.
Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides info...
متن کاملCoherent anti-Stokes Raman scattering for label-free biomedical imaging
Coherent anti-Stokes Raman scattering (CARS) has established itself as an imaging technique capable of providing video-rate imaging of biological specimens through vibrational coherence of endogenous molecules. Current techniques predominantly involve the application of costly, invasive and potentially non-specific dyes or labels for imaging biomolecules. CARS microscopy can however provide a h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: eLight
سال: 2023
ISSN: ['2662-8643']
DOI: https://doi.org/10.1186/s43593-022-00038-8